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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Aug 14;34(10):2254–2260. doi: 10.1161/ATVBAHA.114.304027

Figure 4. Immunoblot analysis of human T/T lipoprotein-free plasma fraction.

Figure 4

Pooled plasma samples from human subjects homozygous for the T/T allele was adjusted to 1.21 g/ml with NaBr and subjected to density ultracentrifugation. The lipoprotein-free bottom was collected, dialyzed against PBS and immunoprecipitated with anti-apoA-V IgG. The precipitated proteins were solubilized in sample treatment buffer, electrophoresed under non-reducing conditions, transferred to a PVDF membrane and probed for apoA-V as described in Materials and Methods. Lane 1, recombinant apoA-V standard (1.5 ng); lane 2, T/T sample immunoprecipitate (molecular weights indicated on left).