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. 2014 Sep 29;206(7):855–865. doi: 10.1083/jcb.201407087

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Figure 1. — Expression of Plk1TD in Emi1-d U2OS cells induces disengagement of short procentrioles that gradually biochemically mature. (A) EM analysis of an Emi1-d Plk1TD-expressing U2OS cell with newly disengaged DCs. Four C1-GFP signals indicate the presence of four centrioles. EM analysis of the same centrioles reveals two longitudinally sectioned DCs (∼260 nm DC1 and ∼220 nm DC2) in the vicinity of two MCs in cross section. (B and C) Localization of Centrobin, Cep152, Cep164, and Odf2 on the centrioles of Emi1-d cells, with or without Plk1TD expression. (D) Quantification of the data presented in B and C. n = 300, from three independent experiments. (E) Multiple microtubule nucleation sites, associated with C1-GFP (yellow arrows), form after nocodazole washout, which indicates that disengaged short centrioles organize functional PCM. Error bars represent the means and SD.