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. 2014 Sep 29;206(7):855–865. doi: 10.1083/jcb.201407087

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Figure 2. — CLEM analysis of centrioles in Emi1-d Plk1TD-expressing cells 70 h after Emi1 depletion and 46 h after Plk1TD expression cells that were fixed and analyzed by EM. (A) 200-nm-thick z sections through centrosomal content of the cell. (B) EM analysis of the cell from A: two ∼500-nm-long MCs are in cross section through eight 80-nm-thick sections (S1–S8). Both MCs are associated with distal (white arrows) and subdistal (green arrows) appendages, and with a short DC (yellow arrows). Disengaged short DCs are marked with gray arrows. Red and yellow asterisks mark the same centrioles, for easier comparison of sections between A and B. (C) Two serial sections show several generations of centrioles; original MCs are sectioned longitudinally. Shorter disengaged DCs are unduplicated (blue arrows) or associated with a procentriole (yellow arrows). (D) Mean centriole length measured from electron micrographs. Error bars represent the means and SD. (E) Selected enlarged images showing short disengaged centrioles with appendage-like structures associated with microtubules (closed arrows) or engaged with a DC (green arrows). The right centriole is the enlarged centriole from C.