Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 29;206(7):855–865. doi: 10.1083/jcb.201407087

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Plk1 promotes premature recruitment of appendage proteins on the centrioles in HU-arrested and cycling cells. (A) HeLa cells arrested in S phase by HU contain only one centriole associated with appendage proteins Cep164, Odf2, FBF1, SCLT1, and ccdc41. Expression of Plk1TD results in accumulation of these proteins on the other MC. (B) Quantification of the data presented in A. n = 300, from three independent experiments. (C) Two G1 cells expressing Plk1TD with all centrosomes associated with Cep164 (left) and Odf2 (right). Plk1TD is still visible on the midbody (MB) but degraded from the centrioles. (D) Examples of Plk1TD-expressing MCF10A cells with one or two cilia labeled for polyglutamylated tubulin and ciliary membrane protein ARL13b. (E) Percentage of ciliated cells with one or more cilia in Plk1TD-negative and -positive cells. n ≥ 100. Exp, experiment.