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. 2014 Sep 15;37(9):685–690. doi: 10.14348/molcells.2014.0179

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The Korean Society for Molecular and Cellular Biology. All rights reserved.

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Fig. 1. — Expression of CPE is enhanced during osteoclast differentiation. BMMs were cultured in M-CSF (10 ng/ml) and RANKL (20 ng/ml) for the indicated number of days. (A) Expression of CPE was determined by RT-PCR. (B) CPE expression was analyzed by immunoblotting. TRAP or cathepsinK (CathK) served as positive controls for osteoclastogenesis, and GAPDH or β-actin served as loading controls. (C) BMMs or osteoclasts (OC) were fixed and permeabilized prior to staining. F-actin and CPE were stained with TRITC-conjugated phalloidin (red) and anti-CPE antibody (green), respectively.