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. 2014 Sep 18;6(9):2787–2803. doi: 10.3390/toxins6092787

Figure 2.

Figure 2

ζ toxin phosphorylates in vitro a fraction of UNAG. Samples containing 2 mM UNAG and 0.5 mM ATP were incubated in the absence (A) or presence (B) of ζ toxin (0.5 µM) for 60 min at 30 °C in buffer B. The reaction products were analyzed by mass spectroscopy. The peaks corresponding to relevant products (ATP, ADP, UNAG and UNAG-3P) are indicated. The intensity (×104) was expressed in arbitrary units (a.u.). In panel B, the 675 to 740 m/z section is enlarged in the insert.