Table 1.
Strains | Relevant genotype | Reference |
---|---|---|
BG687 a | +xylR, PxylA, cat | [12] |
BG689 a | +xylR, PxylA ζY83C, cat | [12] |
BG1127 a | +lacI, Phsp, spc, [pCB799, xylR, PxylA ε, cat] | [12] |
BG1125 a,b | +lacI, Phsp ζ, spc, [pCB799, xylR, PxylA ε, cat] | [12] |
BL21(DE3) c | +[pCB920, PT7 ζ gene, Pω ω and ε genes, bla] | This work |
a The B. subtilis strains are isogenic with BG214 (trpCE metA5 amyE1 ytsJ1 rsbV37 xre1 xkdA1 attSPβ attICEBs1); b BG1125 cells bearing pCB799-borne ε gene were grown in MMS7 containing 0.005% Xyl to titrate basal expression of the wt ζ toxin; c E. coli BL21(DE3) genotype (ompT gal (λ DE3, int::lacI-PlacUV5 T7 gene 1) fhuA2 (dcm) ΔhsdS).