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. 2014 Sep 23;6(9):2840–2856. doi: 10.3390/toxins6092840

Figure 5.

Figure 5

Monocytes pre-treated with the lipids migrate towards the concentrtion gradients of SDF-1α/CXCL12. (A) Monocytes were incubated for 4 h with 20 µM of 9-S-HODE, 9-R-HODE, 13-R-HODE, LPC or media only. The cells were washed and then incubated in the upper wells of Boyden chambers. In the lower wells 0.1, 1, 10 or 100 ng/mL of SDF-1α/CXCL12 was placed; (B) Similar to the panels shown in (A), except that the cells were pre-treated with the lipids for 24 h. Filters were collected, stained and the cells counted. Migration index (MI) was calculated as the numbers of cells migarting in the presence of the chemokine divided by the numbers of cells migrating in the absence of chemokine. Fold increase indicates the increase of MI towards the chemokine after pre-treatment with the lipids vs. the MI obtained towards the chemokine in the absence of lipids pre-treatment (indicated as control = C). Mean ± SEM of 5 experiments performed. p values comparing the effect of lipids versus the controls are shown on top of the columns.