Figure 6.

Oxidized lipids and LPC inhibit IL-6 secretion from monocytes. Monocytes were incubated at a cell concentration of 1 × 10⁶ cells/mL with media or with 200 nM, 2 µM or 20 µM of 9-S-HODE (A); 9-R-HODE (B); 13-R-HODE (C); or LPC (D). After 24 h incubation, the cells were harvested and the cell suspensions were centrifuged and the supernatants were collected. Levels of IL-6 were determined according to the standards provided by the manufacturer. Mean ± SEM of 3 experiments.