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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Mar 25;0:1–19. doi: 10.1016/j.virol.2014.03.002

Figure 10. Persistent HIV-1 infection in astrocytes.

Figure 10

HFA in T-25 flasks were infected with VSV-NLENY1 virus and followed for 160 days. HIV-1 infection was monitored by YFP expression in infected HFA and p24 secretion in culture supernatants (A, B). (A) VSV-NLENY1- infected HFA showing YFP expression (live). (B) Viral kinetics in long-term (40 to 120 days) HIV-1 infected HFA is shown. Control (NC) is a mock-infected HFA. Alu-HIV-LTR PCR demonstrated HIV viral genome integration in infected HFA from 5 to 111 days after infection (inset B). (C) Persistent infection of HIV-1 in astrocytic (SVGA) cells: SVGA-LTR-GFP reporter cells were infected with HIV-1 NL4-3 and GFP-expressing cells were cloned. Clonal population of persistently HIV-1 (NLE−R+)-infected SVGA-LTR-GFP cells (chronic infection) showing sustained green fluorescence and p24 expression by immunofluorescence. (D) HIV-1 DNA integration by Alu-HIV-LTR PCR in NLE−R+ SVGA-LTRGFP infected cells is shown.