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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Mar 25;0:1–19. doi: 10.1016/j.virol.2014.03.002

Figure 8. Robust HIV-1 late gene expression in astrocytes.

Figure 8

(A) HFA cultured for 48 h were infected with VSV-pseudotyped NL4-3 virus (200 ng/ mL p24) and, 48 h after infection, were immunostained for GFAP, p24 and nuclei. Images were captured under a fluorescence microscope (inset). Cells positive for both GFAP (red) and p24 (green) were randomly counted in 10 fields with total nuclear counts (blue) in duplicate sets; mean ± SEM were plotted using a sigma plot. (B) VSV-pseudotyped HIV-1 infection in SVGA-LTR reporter cells: Stable SVGA-LTR GFP or LTR-gagGFP-RRE reporter cells were seeded overnight in six-well culture plates. Next day, cells were infected with VSV-NLE−R+ virus for 2 h (200 ng/ mL p24) and washed. At 48 h after infection, cells were immunostained for p24 (red) and nuclei (blue). Dual-positive cells (green and red) were counted in ten random fields in duplicate cultures and mean positive cells ± SEM were plotted.