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. 2014 Oct 1;127(19):4213–4224. doi: 10.1242/jcs.151167

Fig. 2.

Fig. 2.

Ex vivo cells that lack β2 integrin have podosome formation defects. (A) Resident lung cells (>95% alveolar macrophages) collected from wild type (WT) or Itgb2-null mice by bronchoalveolar lavage were plated on coverslips and stained for β2 integrin (green; FITC), F-actin (red; Alexa-Fluor 555) and vinculin (grey; Alexa-Fluor 633). Images were acquired as described for Fig. 1. Scale bars: 5 µm. (B) Adherent cells were scrutinized for podosome formation using systematic scanning of the coverslips. Quantification of the percentage of cells with podosomes indicated a strong defect in podosome formation in lung-derived cells (**P = 0.001, unpaired t-test). (C) Cellular composition of bronchoalveolar lavage in WT and Itgb2-null mice was determined morphologically by differential counts of DiffQuik-stained cytospin preparations.