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. 2014 Oct 1;127(19):4225–4233. doi: 10.1242/jcs.151613

Fig. 5.

Fig. 5.

STAG2 promotes faithful chromosome segregation. (A) Anaphase in STAG2-deficient H4 cells expressing GFP only (GFP) or GFP-tagged Kif2b (GFP–Kif2b), showing GFP fluorescence (green), centromere staining (red) and DNA (blue). The arrow indicates a lagging chromosome. Scale bar: 5 µm. (B) Quantification of the percentage of anaphase cells with lagging chromosomes in H4 cells overexpressing GFP, GFP–Kif2b, GFP–MCAK or GFP–STAG2. Data show the mean±s.e.m. (n≥300 cells); *P≤0.01. (C) Model of STAG2 activity during mitosis. STAG2 promotes the localization and activity of Bub1 and Aurora B kinase to promote the destabilization of kMT attachments and enhance error correction. In the absence of STAG2, Bub1 and Aurora B kinase do not localize properly, causing hyperstabilization of kMT attachments and reduced error correction efficiency.