Fig. 8.

Morpholino knockdown of Coro1C and RCC2 leads to migration defects in the developing zebrafish. (A) Coro1C and RCC2 expression in morpholino (Mo)-injected whole zebrafish lysate at 3 dpf relative to control morpholino. (B) Lateral views at 32 hpf of Fli1:eGFP transgenic embryos, which exhibited altered migration of neural crest in the first and second pharyngeal arch elements (red dotted line), leading to mixing of the two arches (red arrows), upon injection of Coro1C (23/32 fish) or RCC2 (24/28 fish) morpholinos. (C) col2a1BAC:mCherry transgenic embryos injected with control (n = 49), Coro1C (n = 31) or RCC2 (n = 32) morpholinos, ventral views at 5 dpf, white arrow shows mislocalized chondrocytes between the Meckel's cartilage (mc) and ceratohyal (ch), yellow arrow shows misaligned elements. An enlargement of the boxed area is shown in C′. (D) Alcian Blue and Alizarin Red stained larvae at 5 dpf, ventral views (n = 28–32). (E–G) Position of Sox10:eGFP-expressing neural crest cells at 32 hpf in fish injected with control, Coro1C or RCC2 morpholinos plus rescue mRNA as appropriate. Scale bars: 100 µm.