Table 1.

Comparison of forces involved in the interactions of thrombin with allosteric ligands.

Ligand	Z × Ψ	ΔGionica	KD,nonionic	ΔGnonionicb	Reference
		kcal/mol	mM	kcal/mol
Hirudin peptide (Hir55–65)	1.06	−1.19c	nad	na	19
Thrombomodulin with CSe	4.8	−5.77	0.0125	−6.66	19
Thrombomodulin without CS	2.2	−2.34	0.000708	−8.36	19
Glycocalicin (Soluble GPIbα)	4.2	−4.73	2.88	−3.45	20
Glycocalicin (Soluble GPIbα)	4.6	−5.17c	na	na	21
Heparin	4.8	−5.4	53.3	−1.73	16
SbO4L	−4.85 ±0.43 f	−5.45	2.14 ±0.26	−3.64	this work

^afree energy of binding at 150 mM NaCl arising from ionic interactions.

^bfree energy of binding at 1 M NaCl arising from non-ionic interactions.

^ccalculated from slope measured by authors.

^dnot available.

^echondroitin sulfate.

^fstandard error obtained from linear regression.