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. 2014 Sep 29;2:38. doi: 10.3389/fbioe.2014.00038

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Copyright © 2014 Muller, Marzi and Nicassio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential expression analysis. (A) A scatter plot shows the expression level of canonical miRNAs in the normal and tumor samples. Data have been normalized in reads-per-million (RPM) and shown in log₁₀ scale. Blue and red dots identify those miRNAs with >1 log₂ fold regulation between the two samples. (B) Comparison of the fold change as measured by RNA-seq or by RT-qPCR (miScript assay, Qiagen) for eight randomly selected miRNAs (four upregulated and four downregulated). Three asterisks mark non-concordant results. N.D., no PCR data.