Figure 1.

FOXL2 is necessary for FSH gene expression in vivo. A, Immunohistochemistry of sectioned pituitary glands collected from wild-type (WT) and FOXL2-null mice (KO) at embryonic 18.5 (e18.5) for luteinizing hormone (LH), follicle-stimulating hormone (FSH), common α-subunit αGSU, growth hormone (GH), thyroid-stimulating hormone (TSH), and adrenocorticotropin (ACTH) with antibodies obtained from the National Hormone and Peptide program, reveals reduced levels of gonadotropin hormones in FOXL2-null mice. B, To determine cell numbers, five sections per animal and three animals per group were counted and the difference in cell numbers between genotypes was analyzed by the t-test.