Figure 2.

FOXL2-null mice have lower expression of gonadotrope-specific mRNAs. Quantitative PCR of e18.5 pituitaries shows that gonadotropin gene expression is significantly reduced in FOXL2-null mice. Total RNA was purified from three samples per WT or KO group, each with five pooled pituitaries, reverse transcribed, and the level of hormone expression assayed by real-time PCR. In each sample, the amount of hormone mRNA, calculated from the standard curve, was compared to the amount of Gapdh, and presented as a ratio. * indicate significant difference in the expression in the FOXL2-null animals from the wild-type animals.