Figure 5.

FBE1 and FBE3 bind FOXL2. EMSA was used to analyze the binding of FOXL2 to elements FBE1 and FBE3, which contribute to the induction by FOXL2. A, EMSA with nuclear extracts using 30 bp probes that encompass FBE1 and FBE3. “C” above the lanes designates extracts from control, vehicle-treated LβT2 cells, while “A” designates 2-h activin treatment. Antibodies to FOXL2 “αL2” were included in the binding reaction to identify FOXL2 containing complex (supershift, ss), and specificity determined by comparison to nonspecific IgG antibody (Ig). B, EMSA with in vitro transcribed and translated vector control (V) and FOXL2 (F) with 30 bp probes that encompass FBE1 or FBE3.