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. 2014 Sep 22;8:1481–1497. doi: 10.2147/DDDT.S68818

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© 2014 Karimian et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

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Immunofluorescence analysis.

Notes: An immunofluorescence analysis of (A) Bax and (B) Bcl-2 in MCF-7 cells depicted a marked elevation in the fluorescent intensity of Bax and reduction in the fluorescent intensity of Bcl-2 antibodies conjugated to FITC, in a time-dependent manner. (C) Quantitative analysis of Bax and Bcl-2 expression in MCF-7 cells showed a time dependent increase in the Bax expression and decrease in the Bcl-2 expression at the protein level. *P<0.05.

Abbreviation: FITC, fluorescein isothiocyanate.