Table I.
TaqMan® assays used for reverse transcription quantitative polymerase chain reaction gene expression validation.
| Gene symbol | Gene name | Assay identity or sequence |
|---|---|---|
| Hif1α | Hypoxia inducible factor 1α | Mm00468879_g |
| Bnip3 | BCL2/adenovirus E1B 19 kDa interacting protein 3 | Mm01275601_g |
| Gstz1 | Glutatione S-transferase | Mm01296880_m1 |
| Gsr | Glutatione reductase | Mm00439154_m1 |
| Xbp1 | X-box binding protein 1 | Mm00457357_m1 |
| Nfe2l2 (Nrf2) | Nuclear factor (erythroid-derived 2)-like 2 | Mm00477784_m1 |
| Egr1 | Early growth response protein 1 | Mm00656724_m1 |
| Fos | FBJ osteosarcoma oncogene | Mm00487425_m1 |
| Akap6 | A kinase (PRKA) anchor protein 6 | Mm01292745_m1 |
| Atp8a2 | ATPase, aminophospholipid transporter, class I, type 8A, member 2 | Mm00443740_m1 |
| Entpd4 | Ectonucleoside triphosphate diphosphohydrolase 4 | Mm00491888_m1 |
| Hprt1 | Hypoxanthine phosphoribosyltransferase 1 | F 5′-GCAGTACAGCCCCAAAATGG-3′, R 5′-AACAAA GTCTGGCCTGTATCCAA-3′, probe (VIC) 5′-VIC-AAGCTTGCTGGTGAAAAGGACCTCTCG-3′ |
F, forward; R, reverse.