Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 1;33(10):652–666. doi: 10.1089/dna.2014.2366

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2014, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 3. — Tazarotene-induced activation of the caspase-8-truncated Bid (tBid) cascade and the effects of the caspase-8 inhibitor Z-IETD-FMK in BCC cells. (A) The cells were treated with indicated concentrations of tazarotene for 24 h. Total cell lysates were prepared, resolved by SDS-PAGE, and immunoblotted with the indicated antibodies to detect the non-cleaved and cleaved forms of caspase-8, Bid, and truncated Bid. (B) The bands were analyzed by ImageJ and normalized to actin. The means±SD of the three independent experiments are presented in the bar graph. ^nsp>0.05, *p<0.05, **p<0.01, and ***p<0.001, compared with the 0.1% DMSO-treated group. (C) The reverse effect of the caspase-8 inhibitor on 100 μM tazarotene-induced reduction in cell viability. The data represent the means±SD from three wells. The data are representative of three independent experiments with similar results. (D) The sub-G1 population, (E) caspase and (G) Bid cleavage, and cytosolic cytochrome c expression are presented. (F, H) The bands were analyzed by ImageJ and normalized to actin. The means±SD of the three independent experiments are presented in the bar graph. ^#p<0.05, ^##p<0.01, and ^###p<0.001, compared with the 100 μM tazarotene-treated group.

FIG. 3. — Tazarotene-induced activation of the caspase-8-truncated Bid (tBid) cascade and the effects of the caspase-8 inhibitor Z-IETD-FMK in BCC cells. (A) The cells were treated with indicated concentrations of tazarotene for 24 h. Total cell lysates were prepared, resolved by SDS-PAGE, and immunoblotted with the indicated antibodies to detect the non-cleaved and cleaved forms of caspase-8, Bid, and truncated Bid. (B) The bands were analyzed by ImageJ and normalized to actin. The means±SD of the three independent experiments are presented in the bar graph. ^nsp>0.05, *p<0.05, **p<0.01, and ***p<0.001, compared with the 0.1% DMSO-treated group. (C) The reverse effect of the caspase-8 inhibitor on 100 μM tazarotene-induced reduction in cell viability. The data represent the means±SD from three wells. The data are representative of three independent experiments with similar results. (D) The sub-G1 population, (E) caspase and (G) Bid cleavage, and cytosolic cytochrome c expression are presented. (F, H) The bands were analyzed by ImageJ and normalized to actin. The means±SD of the three independent experiments are presented in the bar graph. ^#p<0.05, ^##p<0.01, and ^###p<0.001, compared with the 100 μM tazarotene-treated group.