Figure 4. Mutation of the Rian locus affects expression of nearby genes specifically in Rian^+/−(ma) mice. (A) Relative locations of six genes around Rian on mouse chromosome 12. The sharp corner shows the direction of transcription. The scale bar represents 100 kb. (B) The expressions of six genes flanking Rian was assayed by Q-PCR in brain and ovary from heterozygous mice. In Rian^+/−(ma) mice, expression of Dlk1 and Mirg increased in brain and Meg3, Mirg, and Dio3 increased in ovary. In Rian^+/−(pa) mice, expression of genes adjacent to Rian showed minimal changes.