Fig. 1.

Effects of 27OH-C on SH-SY5Y cell viability, GSH level, and Aβ secretion. (A) SH-SY5Y cells were challenged with 2.5–25 μM 27OH-C for 16 h and cell viability was measured by MTT assay. (B) SH-SY5Y cells (2×10⁴) were challenged with 10 μM 27OH-C for 16 h in the presence or absence of 3 mM NAC. (C) Intracellular GSH and GSSG levels were measured, after treating cells with 2.5–10 µM 27OH-C for 2 h, by the GSH recycling assay and the GSH/GSSG ratio is shown. (D) Protein levels were measured by BCA assay. Results are from three independent experiments. Significant differences were calculated using one-way ANOVA followed by Tukey׳s multiple comparison test, where *P < 0.05, **P < 0.01, and ***P < 0.001.