Fig. 4.

Effects of 27OH-C, LDL, oxLDL, and their lipids on ASMase activity. SH-SY5Y cells were pretreated with LDL, oxLDL, or their lipids (4 μg of protein) for (A) 2 or (B) 16 h. Pretreated or untreated cells were further incubated with 10 μM desipramine, an inhibitor of acid sphingomyelinase, for 1 h before ASMase activity was measured. Results are from three independent experiments (n = 3). Significant differences were calculated using one-way ANOVA followed by Tukey׳s multiple comparison test, where *P < 0.05 and ^#P < 0.01.