Fig. 2.

a SDS-PAGE analysis of serial dilutions of solubilised thylakoids membranes and T. elongatus PSII complexes isolated using the two-step anion-exchange chromatography method and known amounts of a mix of recombinant non-tagged CyanoP, CyanoQ and Psb27 proteins. 100 % level corresponds to 1 µg of Chl and amount in mix refers to amount of each of the proteins. Protein detected by Coomassie Blue staining. Single asterisk indicates migration of AtpA and double asterisk the migration of thioredoxin peroxidase as determined by mass spectrometry. Assignment of PSII subunits was determined through immunoblotting and mass spectrometry. LMM low molecular mass subunits of PSII. b Semi-quantitative immunoblotting analysis to determine CyanoP, CyanoQ and Psb27 levels in thylakoids and PSII