(A) Total TAG content of wild-type, cca1lhy, and CCA1-OX dry seeds. (B)–(F) Total TAG content of wild-type, cca1lhy, and CCA1-OX seedlings at Day 1 (B), Day 2 (C), Day 3 (D), Day 4 (E), and Day 5 (F) cultured under 12-h-light/12-h-dark cycles on half-strength MS medium supplemented with 20 mM sucrose. White bar, wild-type WS; light gray bar, the cca1lhy mutant; dark gray bar, wild-type Col-0; black bar, CCA1-OX. Data in (A) represents a mean value of three repeats ± SD per seed, each measurement contains 20 dry seeds. Data in (B)–(F) represents a mean value of three repeats ± SD per mg fresh weight (FW) of seedlings, per measurement contains 96–122 mg seedlings. Student's t test for *, P<0.05; **, P<0.01.