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. 2014 Sep 29;9(9):e107948. doi: 10.1371/journal.pone.0107948

Figure 4. TGF-β Stimulated CDH2 promoter Dual-luciferase Activity.

Figure 4

(a) Schematic of five pGL2-CDH2 promoter constructs, including part of the CDH first exon (white rectangles), and potential SBE sites (downward arrows). (b,c) Luciferase activity ratios of CDH2 clone #5 and F1/R1 construct treated with or without 5 ng of TGF-β at 0, 2, 8, and 24 hours in HPNE and 293T cells. **P<0.01, ***P<0.001. (d) The second SBE sequence in the CDH2 promoter and the Mut2 mutant sequence (G→C). (e) Luciferase activity ratio of Mut2 with or without 5 ng TGF-β at 0, 2, 8, and 24 hours in HPNE and 293T cells.