Figure 7. Phylogenetic trees and MGD comparisons of domains of Vpr between selected X4 and R5 sequences.

A total 8 domains of Vpr were selected based on the known functional property region. Phylogenetic trees were created from a total 18 sequences, which were included 9 sequence of X4 and 9 sequences of R5, based on maximum likelihood algorithm. A simply Python automated the process of generating the hundreds of trees, and each branch distance was calculated between all pairs of X4 and R5 sequences. These groups of distances were tested for a significant difference using a two-tailed student t-test. The MGD, SD and P value of each group are shown. The two most significant comparisons were shown in green while the two least significant ones revealed in purple.