Table 2. Comparison of the Env-V3, Vpr, LTR, and Tat MGD between the X4 and R5 populations.

Group comparison	P value
	Env-V3	Vpr	LTR	Tat
X4/R51	1.33E-45	0.0001401	0.083825	0.518572
Subset2	1.13E-21	0.255	0.398	0.519

MGD of each sequence from the two groups was compared by 2-tailed student t-test¹ and the corrected P value, which was determined by a two-tailed t-test using 1000 random iterations, selecting 8 of 16 X4 sequences and 8 of 87 R5 sequences.² The variances between two groups were tested with the F-test; P<0.05 was considered as unequal variance comparison.