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Early manipulation of JH alters latency to copulation in females in a temporally-dimorphic manner. Females were collected and treated with acetone, methoprene, or precocene immediately following eclosion and then aged for 3, 5, or 8 days prior to being paired with a wild-type male for courtship analysis. Animals were observed until copulation occurred or for 30 minutes. n = 50 animals that copulated for each population. One-Way ANOVA, * p < 0.05, *** p < 0.001.
