Early manipulation of JH in males alters male initiation of courtship and female receptivity in a temporally dimorphic manner. Males were collected and treated with acetone, methoprene, or precocene immediately following eclosion and then aged for 3, 5, or 8 days prior to being paired with a wild-type female for courtship analysis. (A) The number of males that failed to initiate courtship within 10 minutes was determined. (B) The number of females that failed to copulate after 30 minutes was determined. n = 50 animals that copulated for each population, Chi-square with 50 animals copulating and the number corresponding to how many failed to either initiate (A) or copulate (B) with acetone treatment used as the expected values, * p < 0.05, ** p < 0.001.