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Protease sensitivity of G6PT wild-type and glycosylation mutants. Intact microsomes were isolated from COS-1 cells infected with Ad-G6PT, Ad-G6PT-p.T53N, or Ad-G6PT-p.S55N (Pan, Chen, Lee, & Chou, 2009). (A) Western blot analysis of the synthesis of G6PT, G6PT-p.T53N, and G6PT-p.S55N. (B) Western blot analysis of G6PT and G6PT-p.S55N after limited trypsin digestion in the absence and presence of tunicamycin.
