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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Genes Immun. 2012 May 24;13(6):481–488. doi: 10.1038/gene.2012.22

Figure 2.

Figure 2

Polarizing cytokine requirements for expression of Ifng, Il17a, and IL26 by effector CD4 T cells. CD4 T cells were stimulated with anti-CD3 and anti-CD28 in the presence of the indicated cytokines. After 5 days, cultures were harvested and restimulated with PMA and ionomycin for 6 hrs. Cultures were harvested and transcript levels of (a) Ifng, (b) Il17a, and (c) IL26 were determined as described in Figure 1. Results are expressed relative to cultures stimulated with anti-CD3 and anti-CD28 in the absence of additional cytokines. (d) Human CD4+ Th1 cells (Hu CD4) or murine BAC-transgenic CD4+ Th1 cells (Mu CD4) were stimulated with PMA/Ionomycin. Cultures were harvested and IL26 transcript levels determined as in Figure 1. (e) Murine BAC-transgenic splenic or colonic NK cells (DX5+) were stimulated for 48 hr. Transcript levels of Ifng, IFNG, and IL26 were determined as described in Figure 1. Results are expressed relative to Gapdh transcript levels. Error bars are S.D. * = not detected.