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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Genes Immun. 2012 May 24;13(6):481–488. doi: 10.1038/gene.2012.22

Figure 5.

Figure 5

Transcriptional enhancer activity of CNS-77. (a) Schematic of the IFNG-luciferase reporter construct with and without CNS-77 inserted into the Pac1 cloning site. (b) Purified CD4+ T cells were transfected with IFNG-luciferase reporter constructs. After 24 hrs, transfected cells were stimulated with PMA and ionomycin for an additional 24 hrs. Luciferase activity in cell lysates was determined and results are expressed as relative luciferase activity. (c) Purified Jurkat T cells were nucleofected with the indicated reporter constructs and cultured and stimulated as in (b). Luciferase activity in cell lysates was determined and results are expressed as relative luciferase activity. (d) Peripheral human CD4+ T cells were stimulated with anti-CD3 and anti-CD28 for 4 hours and NF-κB binding to CNS-77 was determined by chromatin immunoprecipitation assay. Error bars are S.D.