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RNA polymerase II occupancy across the IL26-IFNG genomic region is dependent upon CNS-30. (a) Average crosslinking frequency between Ifng (indicated by the open bar with dashes) and the indicated genomic positions in nuclei from effector Th1 and Th2 cells. (b & c) RNA pol II recruitment to the indicated genomic positions in (b) BAC WT and (c) ΔCNS-30 transgenic Th1 and Th2 cells determined by ChIP assay. Results are expressed as fraction of input. Error bars are S.D.
