Table 1. Primers used for qPCR expression analysis of threespine stickleback genes.

Target	Gene name	Ensembl gene ID	Primer sequences	°C	E (%)	Reference
ATP1A3b	ATPase, Na+/K+ transporting, alpha 3b polypeptide	ENSGACG00000009524	F: AGCCGAGATCCCCTTCAACTCCA	60	99.07	This study
			R: GCTCCTTCCCCTGCACCAGGA
CFTR	Cystic fibrosis transmembrane conductance regulator	ENSGACG00000009039	F: GCAGGCCTCTTCTTCACCAA	58	98.51	McCairns et al. (2009)
			R: TCCAGATAGAGGCTGATGTTCTTG
KCNH4	Potassium voltage-gated channel subfamily H member 4	ENSGACG00000008648	F: CACAGTGACCTCTCTGGTGC	60	99.29	This study
			R: AGACATGAGCAGGGTCAGGA
HSP70	Heat shock protein 70	ENSGACG00000013048	F: ATCGGTATTGACCTGGGCAC	60	99.20	This study
			R: GGTATCGGTGAACGCCACAT
Reference
EF1α	Elongation factor 1	ENSGACG00000002182	F: CATTGTCACTTACCTGAATCACATGA	60	99.26	McCairns et al. (2009)
			R: TGTGGCATTTAACAACATTTCCA
GADPH	Glyceraldehyde-3-phosphate dehydrogenase	ENSGACG00000005864	F: CAAACCGTTGGTGACAGTATTTG	60	99.9	Sanago et al. (2011)
			R: GCACTGAGCATAAGGACACATCTAA