Skip to main content
. 2014 Sep 29;9(9):e108135. doi: 10.1371/journal.pone.0108135

Figure 2. Rhodopsin transcript and protein levels in wild type and knockin mouse lines.

Figure 2

A. Rhodopsin mRNA in wild type mice (mRho+/+), hRho-GFP homozygous mice (hRhoG/hRhoG), ID2-hRho-GFP homozygous (ID2/ID2) and heterozygous (ID2/+) mice, and Q344X-hRho-GFP homozygous (Q344X/Q344X) and heterozygous (Q344X/+) mice. Northern blot analysis used radiolabeled probes against human rhodopsin cDNA to detect human and mouse rhodopsin mRNA. Lane 1 shows the five species of mouse rhodopsin mRNA generated from the normal mouse rhodopsin locus. Lanes 2, 3, and 5 show the seven species of human rhodopsin mRNA generated from the knockin alleles. Lanes 4 and 6 show the mixture of species produced in heterozygous mice. Radioactive bands were quantified using a PhosphorImager and normalized for loading using the intensity of the 28S ribosomal rRNA bands in agarose gel before transfer (bottom). Marker sizes at the right of the gel are given in kilobases. B. Protein quantification by immunoblot of retina lysates probed with antibodies directed against the N-terminus of rhodopsin (mAb B630-N). Nine retinas were analyzed for each genotype. C. Total rhodopsin per retina as quantified by difference spectrophotometry. At least three retinas per genotype were analyzed.