Fig. 4.

Lipid composition of VACV. Viral lipids were extracted in the presence of endogenous lipid standards. Lipid analysis was performed by nanoelectrospray ionization tandem mass spectrometry, employing lipid class-specific scan procedures. Due to the low abundance of PG and/or LBPA an unequivocal identification as either PG or LBPA via the mass spectrometric fragmentation pattern was not possible.

A. Lipid profile of VACV displayed as mol% of measured lipids.

B. Lipid distributions (in mol%) displayed in class categories.

aPC, diacyl-phosphatidylcholine; ePC, ether-acyl-phosphatidylcholine; SM, sphingomyelin; aPE, diacyl-phosphatidylethanolamine; ePE, ether-acyl-phosphatidylethanolamine; pl-PE, plasmalogen-phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositol; DAG, diacylglycerol; TAG, triacylglycerol; CE, cholesterolester; Chol, cholesterol; LPC, lyso- phosphatidylcholine; PG, phosphatidylglycerol; LBPA, lysobisphosphatidic acid; HexCer, hexosylceramide; Cer, ceramide; PA, phosphatidic acid; ST, sterol; PGL, phosphoglycerolipids; SL, sphingolipids; NL, neutral storage lipids.