. 2014 Sep 29;9(9):e107022. doi: 10.1371/journal.pone.0107022

Table 2. Results of quantitative RT-PCR analysis of relative AQP4 mRNA expression in hypoglycemic rat brain cortical tissue (mean ± SD).

Group	△C_T	△△C_T	2^−△△CT
Normal Control	8.5±1.01	0	1
Sham Hypo	8.18±0.32	−0.32±0.32	1.27±0.27
Hypo	7.25±0.9	−1.25±0.90	2.75±1.59
Hypo+R6 h	6.63±0.80	−1.87±0.80	3.28±1.15
Hypo+R24 h	6.42±0.56	−2.09±0.56	4.53±1.88^#
Hypo+R3 d	6.42±0.38	−2.08±0.38	4.33±1.11^#

Levels of AQP4 mRNA were upregulated after 24 h of recovery compared to the Sham Hypo group (p<0.05). # p<0.05 (Dunnett's method, n = 5). CT: cycle threshold for the gene. SD: standard deviation.