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Competitive interaction between NCS-1 and CaM for binding to GST-PQ. (A) NCS-1 (5 μM) was incubated with 5 μM GST-PQ in the presence of varying concentrations of CaM. Bound NCS-1 and CaM were visualized by Western blotting with specific antibodies. (B) Data from panel A were quantified by densitometry, and NCS-1/CaM bound to GST-PQ was plotted as a function of the CaM concentration present in the binding assay.
