Figure 1.

Proteomic analysis of galectin-3 binding ligands purified from seminal plasma. Affinity-purified, galectin-3 binding proteins were separated by SDS-PAGE and stained with Coomassie blue. Protein bands were excised, and the isolated proteins were identified by MS/MS. Spectral counts for identified proteins were calculated, and proteins with 10% or greater of the total spectral count for each band are shown in order of increasing percent spectral count.