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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Am J Reprod Immunol. 2014 May 23;72(4):403–412. doi: 10.1111/aji.12273

Figure 4.

Figure 4

Co-migration of galectin-3-reactive and immunoreactive spots on 2D electroblots of seminal plasma. Clarified seminal plasma was subjected to 2D immunoblot and galectin-3 lectin blot analysis. Three distinct immunoreactvie spot patterns ~30 kDa/~pI 7, ~50 kDa/~pI 5, and ~40 kDa/~pI 5 were identified by galectin-3 lectin blot analysis. These three distinct galectin-3-reactive spot patterns comigrated with the immunoblot spot patterns for PSA, PAP, and ZAG at their expected apparent molecular weight and pI.