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. 2014 Sep 20;12:90. doi: 10.1186/1477-7827-12-90

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© Lee et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Testosterone, but not 1,25D ₃ , enhanced ERβ expression; testosterone and 1,25D ₃ had no effect on VDR expression. Granulosa cells were pretreated with 1,25D₃ for 15 min and subsequently treated with one of two testosterone doses or vehicle for 24 h (n = 4–5 in each group). The granulosa cell lysates were subjected to western blot analysis for (A) ERβ and (B) VDR expression levels. The levels of ERβ and VDR expression were quantified using densitometry and normalized to β-actin. The values represent the means ± SEM. **, p < 0.005 for comparison with the vehicle-treated group. #, p < 0.05 for comparison of two drug groups. The values listed within column indicate the means.