. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: Chem Sci. 2014 Jul 4;5(10):4076–4081. doi: 10.1039/C4SC01162G

Table 1.

Melting temperature (Tm) for DNA duplexes containing F base.^a

Entry	5′-d(GCGTACXCATGCG) 3′-d(CGCATGYGTACGC)
Entry	X-Y	Tm (°C)
1	A-T	61.5
2	C-G	62.4
3	F-F	51.5
4	F-A	50.1
5	F-G	48.8
6	F-C	47.2
7	F-T	49.1
8	A-F	50.4
9	G-F	45.7

Conditions: 1 μM DNA, 100 mM NaCl, 10 mM MgCl₂, 10 mM PIPES, pH 7.0. The heating rates were 0.5 °C/min.