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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biomaterials. 2014 Jun 21;35(28):8175–8185. doi: 10.1016/j.biomaterials.2014.05.073

Fig. 3.

Fig. 3

Hypoxia and HIF1α mediated the uptake of MHI-148 dye by tumor xenografts. (A) Representative in vivo and ex vivo NIRF images of control (left flank) vs. HIF1α-overexpressing (right flank) (left panel) and control (left flank) vs. HIFIα-knockdown (right flank) (right panel) PC-3 tumor xenografts. Scale bars represent x108 for both in vivo and ex vivo NIRF in the unit of radiant efficiency. (B) Quantitation of tumor uptake of MHI-148 dye (N=5, mean ± SEM) presented as the ratio of dye intensity to tumor weight. *p<0.05, **p<0.01. (C) IHC analysis of HIF1α and VEGF-A expression in PC-3 tumor xenografts with different manipulation of HIF1α levels as indicated. Representative images are shown. Original magnification, x400; scale bars represent 20 µm.