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. 2014 Sep 16;2:137. doi: 10.1186/s40478-014-0137-3

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© Hiniker et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mitochondrial respiratory chain immunofluorescence. Immunofluorescence staining (green) for Complex I (30 kD NDUFS3) [(a) and (g)], Complex II (30 kD FeS protein) (b), Complex III (core 2) (c), Complex IV-I (IV subunit I) (d), Complex IV-IV (IV subunit IV) [(e) and (h)], and Complex V (OSCP) (f); immunofluorescence staining for porin (red) is a reference control for mitochondrial density and distribution. Arrows, examples of partially deficient fibers; arrowheads, examples of totally deficient fibers; asterisk, an example of the area with fibers showing mitochondrial hypodensity. Original magnification: a-f, 100x; g-h, 400x.