Table 4. Densitometry measurements in zymography and reverse zymography gels.
CΟ (n = 10) | ΑΤ (n = 10) | EX (n = 10) | ΑΤ+EX (n = 10) | ANOVA, post-hoc p values | |
MMP-2 (pg) | 81.3±11.1 | 61.0±8.7 | 100.3±12.2 | 81.8±10.4 | P2<.001 P3 = .001 P4 = .004 |
p<.001 | p = .003 | p = .931 | PA<.001 | ||
pro-MMP-2 (pg) | 102.2±12.0 | 83.1±12.0 | 86.4±10.9 | 81.3±13.8 | P2 = .943 P3 = .988 P4 = .813 |
p = .003 | p = .009 | p = .003 | PA = .003 | ||
MMP-9 (pg) | 113.5±13.9 | 92.3±10.3 | 97.1±7.5 | 89.1±10.0 | P2 = .772 P3 = .921 P4 = .395 |
p = .002 | p = .006 | p<.001 | PA<.001 | ||
TIMP-1 (peak area) | 3002±357 | 3253±402 | 3903±562 | 4045±472 | P2 = .015 P3 = .002 P4 = .897 |
p = .158 | p<.001 | p<.001 | PA<.001 | ||
TIMP-2 (peak area) | 2356±324 | 1932±336 | 2159±307 | 1905±233 | P2 = .352 P3 = .997 P4 = .258 |
p = .010 | p = .180 | p = .002 | PA<.006 |
Values for MMP-2, MMP-9 and proMMP-2 are given in units of picograms of enzyme per homogenized sample. Values for TIMP-1 and TIMP-2 are given as densitometry measurements of peak area per homogenized sample.
p: p values of differences in variables between each experimental group and control group; PA: significance of variable differences between all groups (one-way ANOVA); P2: significance of differences in variables between groups AT and EX (post-hoc Tuckey HSD test); P3: significance of differences in variables between groups AT and AT+EX (post-hoc Tuckey HSD test); P4: significance of differences in variables between groups EX and AT+EX (post-hoc Tuckey HSD test).