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. 2014 Sep 29;9(9):e108240. doi: 10.1371/journal.pone.0108240

Table 4. Densitometry measurements in zymography and reverse zymography gels.

CΟ (n = 10) ΑΤ (n = 10) EX (n = 10) ΑΤ+EX (n = 10) ANOVA, post-hoc p values
MMP-2 (pg) 81.3±11.1 61.0±8.7 100.3±12.2 81.8±10.4 P2<.001 P3 = .001 P4 = .004
p<.001 p = .003 p = .931 PA<.001
pro-MMP-2 (pg) 102.2±12.0 83.1±12.0 86.4±10.9 81.3±13.8 P2 = .943 P3 = .988 P4 = .813
p = .003 p = .009 p = .003 PA = .003
MMP-9 (pg) 113.5±13.9 92.3±10.3 97.1±7.5 89.1±10.0 P2 = .772 P3 = .921 P4 = .395
p = .002 p = .006 p<.001 PA<.001
TIMP-1 (peak area) 3002±357 3253±402 3903±562 4045±472 P2 = .015 P3 = .002 P4 = .897
p = .158 p<.001 p<.001 PA<.001
TIMP-2 (peak area) 2356±324 1932±336 2159±307 1905±233 P2 = .352 P3 = .997 P4 = .258
p = .010 p = .180 p = .002 PA<.006

Values for MMP-2, MMP-9 and proMMP-2 are given in units of picograms of enzyme per homogenized sample. Values for TIMP-1 and TIMP-2 are given as densitometry measurements of peak area per homogenized sample.

p: p values of differences in variables between each experimental group and control group; PA: significance of variable differences between all groups (one-way ANOVA); P2: significance of differences in variables between groups AT and EX (post-hoc Tuckey HSD test); P3: significance of differences in variables between groups AT and AT+EX (post-hoc Tuckey HSD test); P4: significance of differences in variables between groups EX and AT+EX (post-hoc Tuckey HSD test).