Figure 1.

Construction and confirmation of the Δ ga5dh mutant S. suis and its complemented strain by multiplex PCR and Western blot analyses. (A) Genomic DNA extracts from the WT or the deletion mutant were used as templates: WT strain (lanes 1–4) and Δga5dh mutant (lanes 5–9). Two different DNA ladder markers, which are marked as M^a and M^b respectively, were used and labeled. The primer pairs and the theoretical size (bp) of the indicated PCR product are as follows: lanes 1: LU/RD, 2892 bp; 2: ga5dh-F/ga5dh-R, 835 bp; 3: ga5dh-F/spc-R, (-); 4: spc-F/ga5dh-R, (-); 5: LU/RD, 3209 bp; 6: ga5dh-F/ga5dh-R, (-); 7: ga5dh-F/spc-R, (-); 8: spc-F/ga5dh-R, (-); and 9: spc-F/spc-R, 1130 bp. (B) Western blot assay characterizing the protein expression of Ga5DH. The indicated S. suis cells were probed with Ga5DH polyclonal antibodies, and the expression profiles are shown