Table 1.
Strains/ plasmids | Characteristics | Origins |
---|---|---|
Bacterial strains | ||
05ZYH33 | A virulent Chinese isolate of S. suis serotype 2 | Feng et al. (2008); Tang et al. (2006) |
05ZYH33Δga5dh | 05ZYH33 derivative with the ga5dh gene replaced by a spcR gene cassette | This work |
Cga5dh | Complemented strain of 05ZYH33 Δga5dh | This work |
DH5α | An E. coli cloning host | Lab stock |
BL21 | An E. coli expression host | Lab stock |
Plasmids | ||
pUC-18 | Cloning vector | TaKaRa |
pET28b | His-tag fusion expression vector | Novagen |
pET30a | His-tag fusion expression vector | Novagen |
pGEX-6P-1 | GST-tag fusion expression vector | Novagen |
pVA-838 | E. coli - S. suis shuttle vector | Lab stock |