Table 1.
Bacterial strains and plasmids used in this study
| Strains/ plasmids | Characteristics | Origins |
|---|---|---|
| Bacterial strains | ||
| 05ZYH33 | A virulent Chinese isolate of S. suis serotype 2 | Feng et al. (2008); Tang et al. (2006) |
| 05ZYH33Δga5dh | 05ZYH33 derivative with the ga5dh gene replaced by a spcR gene cassette | This work |
| Cga5dh | Complemented strain of 05ZYH33 Δga5dh | This work |
| DH5α | An E. coli cloning host | Lab stock |
| BL21 | An E. coli expression host | Lab stock |
| Plasmids | ||
| pUC-18 | Cloning vector | TaKaRa |
| pET28b | His-tag fusion expression vector | Novagen |
| pET30a | His-tag fusion expression vector | Novagen |
| pGEX-6P-1 | GST-tag fusion expression vector | Novagen |
| pVA-838 | E. coli - S. suis shuttle vector | Lab stock |